QIAGEN's real-time PCR cycler, the Rotor-Gene Q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands. Together with optimized QIAGEN kits for real-time PCR, the Rotor-Gene Q enables streamlined analysis for a wide range of applications.
Q-Rex Software is operating and analysis software for life science qPCR applications. A choice of plug-ins lets you add new functionality without affecting established workflows.
Features
• Outstanding thermal and optical performance due to rotary format
• An unmatched optical range spanning UV to infrared wavelengths
• State-of-the art analyses supported by user-friendly software
• Low maintenance and maximum convenience due to robust design
• High performance in multiple applications with QIAGEN assays
Precise real-time PCR analysis.
Twofold dilutions of human genomic DNA from 30 ng (10,000 copies) to 0.06 ng (20 copies) were used as a template in real-time PCR. Five replicate reactions were run for each dilution using a self-designed TaqMan® assay for IL1R2 and the Rotor-Gene Probe PCR kit on the Rotor-Gene Q. The average difference in the CT values between all dilutions was 1.07 cycles. Human genomic DNA was used as template in 72 replicate real-time PCRs using a self-designed TaqMan® assay for BCL2 on the Rotor-Gene Q without ROX normalization. The average CT value was 24.94 with a standard deviation of only 0.05, equivalent to a CV of 0.2%.
Performance
The Rotor-Gene Q is the only real-time cycler currently capable of deciphering the most difficult class IV SNPs by HRM. Harness the power of HRM using dedicated QIAGEN HRM Kits for applications such as genotyping (see figure "Identification of a class IV SNP" for data from the Type-it HRM PCR Kit), quantitative methylation analysis (see figure "Highly sensitive detection of methylated DNA" for data from the EpiTect HRM PCR Kit), gene scanning, and sequence matching. The Type-it HRM PCR Kit reliably and accurately detects gene mutations and SNPs. The EpiTect HRM PCR Kit enables fast screening and accurate detection of changes in CpG methylation status of bisulfite converted DNA.
Unique rotary design for outstanding performance
The unique centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currently available (see figure "Cross-section of the Rotor-Gene Q"). Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Detection is similarly uniform. When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis (see figure "Precise real-time PCR analysis"). It also eliminates sample-to-sample variations and edge effects. These are unavoidable in traditional block-based instruments due to temperature gradients across the block and multiple, complex optical pathways.
The rotary design delivers:
• Tube-to-tube variation ±0.02°C
• Uniform detection eliminating the need for ROX reference dyev
• Fast ramping and negligible equilibration times for short run-times
• Complete confidence in your results
Unrivaled optical range enables multiple applications
Whether your assay is based on intercalating dyes such as SYBR® Green, probes such as hydrolysis (TaqMan®), hybridization (FRET), Scorpion probes, or other multiplex chemistries, the Rotor-Gene Q meets your requirements. With up to 6 channels spanning UV to infrared wavelengths, the cycler delivers the widest optical range currently available (see table). In addition, the software allows you to create new excitation/detection wavelength combinations, which means that the Rotor-Gene Q is compatible with dyes you may use in the future.
Channel |
Excitation (nm) |
Detection (nm) |
Examples of fluorophores detected |
Blue |
365 ± 20 |
460 ± 20 |
Marina Blue, Edans, Bothell Blue,
Alexa Fluor 350, AMCA-X |
Green |
470 ± 10 |
510 ± 5 |
FAM, SYBR® Green I, Fluorescein,
EvaGreen, Alexa Fluor 488 |
Yellow |
530 ± 5 |
557 ± 5 |
JOE, VIC, HEX, TET, MAX, CAL Fluor Gold 540, Yakima Yellow |
Orange |
585 ± 5 |
610 ± 5 |
ROX, CAL Fluor Red 610, Cy 3.5,
Texas Red, Alexa Fluor 568 |
Red |
625 ± 5 |
660 ± 10 |
Cy5, Quasar 670, LightCycler
Red640, Alexa Fluor 633 |
Crimson |
680 ± 5 |
712 high pass |
Quasar 705, LightCycler Red705,
Alexa Fluor 680 |
HRM |
460 ± 20 |
510 ± 5 |
SYBR® Green I, SYTO9, LC Green,
LC Green Plus+, EvaGreen |
