Live-cell imaging is a useful tool for monitoring the quality of cell cultures. A simple but effective set-up utilizing a high quality camera allows researchers to evaluate cellular health at regular points in time. The CytoSMART Lux2 duo-kit is a compact and cost-effective method for carrying out this type of analysis, following the complete growth progression of mammalian cells.

The CytoSMART Lux2 Duo Kit is an compact automated system, specifically designed to operate from within CO2- incubators and hypoxia chambers. Two devices operate via a single laptop, saving precious lab space. Gathering real-time insight into the progression of cell growth is now completely non-invasive. Bright-field imaging is deployed to create real time time-lapse videos, accessible remotely. Samples are also imaged under identical conditions, providing a robust platform for unambiguous comparison between cell culture variables, retaining data integrity.

Deploying this dual camera mini live cell imaging system is particularly useful in instances of stem cell research. The remote functionality of the CytoSMART cloud allows researchers to gain insight into the current confluence levels on their phone. This type of microscopy avoids over-growing of cultures or the loss of stemness in the cell-line, ensuring optimal yield. Your team can inspect the colonies that are formed by iPSCs and the morphology of MSCs without setting foot in the lab. Reducing the time spent in the lab will be crucial for many groups that try to continue research while complying to the social distancing rules.

Inspect morphology and analyze cell confluency - Imaging at regular time-intervals allows researchers to make quick decisions around cell quality, as any negative indicators such as blebbing, cell rounding, multi-nucleation and vacuole formation can be easily recognized. Variability in growth speed is easily recognized using the CytoSMART cell monitoring software, while image analysis provides an unambiguous read out for cell confluency meaning you can be confident in your data’s integrity. As such, the system is the ideal approach for the systematic comparison of cultures, in order to select the ideal culturing conditions

Applications -
With the CytoSMART™ Lux2 Duo Kit you have a big advantage over your colleagues and competitors. With our cloud-based solution, you have access to the following application anywhere and anytime you need it:
• Monitor cell division
• Monitor cell growth and confluence (automated image analysis)
• Analyze cell migration and wound healing (scratch assays; automated image analysis)
• Monitoring colony formation
• Time lapse imaging & study chemotaxis
• Study cells cultured inside microfluidic devices
• Study stem cell differentiation
• Cell culture Quality Control

However, you are not limited to these applications or the CytoSMART™ image analysis software. All images and movies can be downloaded from the CytoSMART™ Cloud environment so you can use other (custom) image analysis algorithms if necessary.